Isolation and antimicrobial activity of feruloyl oligosaccharide ester from pineapple stem residues

Ferulic, rho-coumaric and rho-hydroxybenzoic acids, a major components of p henolic acid in pineapple stem were extracted by alkali-treatment, The tota l contents of these alkali extractable phenolic acids were estimated as 8.5 , 15.3, and 8 mg/g of the plant tissues, respectively. Feruloyl oligosaccha ride ester (FE) was released from this plant tissues by the treatment with the crude enzyme of Sporotrichum sp. HG 1. Synergism between purified carbo xymethyl cellulase (CMCase) and xylanase of this strain was observed for th e production of FE from this tissues. The FE was found to be more effective than ferulic acid monomer for bacterial growth. The FE showed inhibitory e ffect against many kinds of gram positive and negative bacteria except Lact obacillus delbrueckii, Streptococcus mutans, S. salivarius, Enterococcus fa ecalis, and E. casseliflavus.