Screening of mitochondrial proteins in winter rye, winter wheat, elymus and maize with an immunochemical affinity to the stress protein 310 kD and their intramitochondrial localization in winter wheat

An investigation of mitochondrial proteins with immunochemical affinity to winter rye CSP 310 was carried out in members of the Graminacea with differ ent tolerances to low temperature - maize, winter wheat and winter rye and the low temperature tolerant wild grass - Elymus sibiricus using PAGE-elect rophoresis. Western blotting showed that among the native mitochondrial pro teins of all species investigated there are proteins with immunochemical af finity to cytoplasmatic CSP 310 with molecular weights 310 kD, about 230 an d about 140-110 kD. Western blotting of submitochondrial fractions showed t hat in outer mitochondrial membrane there are polypeptides with molecular w eights 60 and 58 kD with immunochemical affinity to CSP 310 subunits. In in ner mitochondrial membrane, western blotting showed polypeptides with molec ular weights 66, 60, 55 and 23 kD. In mitochondrial matrix polypeptides wit h molecular weights 56 and 66 kD were found. (C) 2000 Elsevier Science Ltd. All rights reserved.