A mycelial mat of Lentinus edodes was treated with 0.01% (w/v) colchicine s
olution for 240 h at 26 degrees C and autopolyploidization occurred. The my
celia were treated subsequently with the haploidizing reagent, benomyl, and
fanshaped sectors were produced from colonies. Among such sectors, cellula
se hyperproducers could be selected. The cellulase productivity of the hype
rproducer, L1, did not decrease through five generations.