In vivo antigen challenge in celiac disease identifies a single transglutaminase-modified peptide as the dominant A-gliadin T-cell epitope

Celiac disease (CD) is an increasingly diagnosed enteropathy (prevalence, 1 :200-1:300)(1) that is induced by dietary exposure to wheat gliadins(2) (as well as related proteins in rye and barley) and is strongly associated wit h HLA-DQ2 (alpha 1*0501, beta 1*0201), which is present in over 90% of CD p atients(3). Because a variety of gliadin peptides have been identified as e pitopes for gliadin-specific T-cell clones(4-6) and as bioactive sequences in feeding studies and in ex vivo CD intestinal biopsy challenge(7-9), it h as been unclear whether a 'dominant' T-cell epitope is associated with CD. Here, we used fresh peripheral blood lymphocytes from individual subjects u ndergoing shortterm antigen challenge and tissue transglutaminase-treated, overlapping synthetic peptides spanning A-gliadin to demonstrate a transien t, disease-specific, DQ2-restricted, CD4 T-cell response to a single domina nt epitope. Optimal gamma interferon release in an ELISPOT assay was elicit ed by a 17-amino-acid peptide corresponding to the partially deamidated pep tide of A-gliadin amino acids 57-73 (Q65E). Consistent with earlier reports indicating that host tissue transglutaminase modification of gliadin enhan ces gliadin-specific CD T-cell responses(10), tissue transglutaminase speci fically deamidated Q65 in the peptide of A-gliadin amino acids 56-75. Disco very of this dominant epitope may allow development of antigen-specific imm unotherapy for CD.