Recombinant forms of the neurotrophic factor pigment epithelium-derived factor activate cellular metabolism and inhibit proliferation of the RAW macrophage cell line
J. Cohen et al., Recombinant forms of the neurotrophic factor pigment epithelium-derived factor activate cellular metabolism and inhibit proliferation of the RAW macrophage cell line, NEUROIMMUNO, 7(1), 2000, pp. 51-58
Recombinant forms of the neurotrophic factor pigment epithelium-derived fac
tor (PEDF) activate metabolism of RAW macrophage cells while simultaneously
inhibiting their proliferation. The recombinant forms (rPEDF) acted with E
C(50)s of 0.1-1 nM while full-length native bovine PEDF was inactive. Urea,
which is the buffer used to extract recombinant PEDF, stimulated RAW cell
proliferation, the first report of an effect of urea on non-kidney cells. P
EDF acted within 12 h and its effects persisted up to 72 h with continuous
exposure. Although rPEDF had no direct action on glioma cell lines, it incr
eased the amount of a soluble factor released by RAW cells which was capabl
e of blocking glioma cell division. Thus PEDF may function as a neuroimmune
modulator, affecting both neural and immune system cells. Copyright (C) 19
99 S. Karger AG, Basel.