Zx. Wang et al., Costimulatory signal through CD86 is important in Th2 response in Trichinella spiralis infected mice, PARASITE IM, 22(3), 2000, pp. 121-130
In order to study the role of the costimulatory signals in Th2 cytokine pro
duction, monoclonal antibodies (mAbs) against cell adhesion molecules (CAMs
) were added to cultured cells obtained from the mesenteric lymph nodes (ML
Ns) of mice infected with Trichinella spiralis, followed by a determination
of interleukin (IL)-5 and IL-4 in the culture supernatant. IL-5 production
by MLN cells stimulated with somatic antigen was significantly reduced by
addition of anti-CD86 but not by anti-CD80 mAb. Combination of anti-CD80 an
d anti-CD86 mAbs reduced IL-5 production most effectively. IL-4 production
induced by anti-CD3 mAb was suppressed only by the addition of anti-CD86 mA
b. Blockade of the ICAM-1/LFA-1 and VCAM-1/VLA-4 interactions was less effe
ctive on the production of IL-5 and IL-4 than the addition of anti-CD86 mAb
alone. In contrast to the in vitro cytokine production, intraperitoneal in
jection of anti-CD80, anti-CD86 mAb, or both, similarly suppressed the peak
of the eosinophilia on day 21. Elevation of somatic antigen-specific IgE a
nd IgG1 levels as well as total IgE was not inhibited by the administration
of anti-CD80, anti-CD86 mAb or both. In-vitro and in-vivo effects of CTLA-
4 immunoglobulin were similar to those of combined treatment with anti-CD80
and anti-CD86 mAbs. These results suggest that the interaction between ant
igen-presenting cells and CD4 T cells through CD86 are most important in Th
2 response during T. spiralis infection.