A novel method for growing Arabidopsis thaliana plants hydroponically

Citation
Rn. Arteca et Jm. Arteca, A novel method for growing Arabidopsis thaliana plants hydroponically, PHYSL PLANT, 108(2), 2000, pp. 188-193
Citations number
9
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PHYSIOLOGIA PLANTARUM
ISSN journal
00319317 → ACNP
Volume
108
Issue
2
Year of publication
2000
Pages
188 - 193
Database
ISI
SICI code
0031-9317(200002)108:2<188:ANMFGA>2.0.ZU;2-W
Abstract
In this report we describe the development of a simple system for growing i ndividual Arabidopsis plants hydroponically under environmental conditions which are simple to maintain while maximizing growth and development. The s ystem consists of a Life Raft Float Unit with a 14-20 mm sponge inserted in to its center; this unit is placed into a Magenta GA7 vessel containing Ara bidopsis nutrient solution, Plants grown in this experimental system do not require aeration, 125 mu mol m(-2) s(-1) supports vigorous plant growth, 1 /4 to 1/8 strength Arabidopsis nutrient solution promotes maximal growth wh ile higher or lower levels have adverse effects and temperatures between 18 and 24 degrees C were found to be optimal. Plants grown under these condit ions exhibit physiological factors such as time to flowering and overall ap pearance similar to those grown in potting media. The low maintenance metho d described in this paper provides an alternative to existing hydroponic me thods used to grow Arabidopsis. Adaptation of this system for growing large numbers of plants in 1 container is also discussed.