Tj. Jackson et al., Action of the fungicide phosphite on Eucalyptus marginata inoculated with Phytophthora cinnamomi, PLANT PATH, 49(1), 2000, pp. 147-154
Although phosphite has been effective in the control of P. cinnamomi in E.
marginata (jarrah), the biochemical mechanisms behind phosphite protection
are poorly understood. Using an aeroponics system, jarrah clones with moder
ate resistance to P. cinnamomi were treated with foliar applications of pho
sphite (0 and 5 g L-1). The roots were inoculated with zoospores of P. cinn
amomi at 4 days before and 0, 2, 5, 8 and 14 days after phosphite treatment
. Root segments were then analysed for activity of selected host defence en
zymes (4-coumarate coenzyme A ligase [4-CL], cinnamyl alcohol dehydrogenase
[CAD]) and the concentration of soluble phenolics and phosphite. Lesion de
velopment was most effectively reduced when phosphite concentrations within
the roots were highest (i.e. days 8-14). During this time, the levels of h
ost defence enzymes remained relatively unchanged. Lesion development was a
lso effectively restricted when phosphite concentrations within the roots w
ere lowest (i.e. days 2 and 5); a significant increase in host defence enzy
mes was associated with this decrease in lesion development. It was conclud
ed from these studies that the effect of phosphite in controlling the patho
gen is determined by the phosphite concentration at the host-pathogen inter
face. When phosphite concentrations within the roots are low, phosphite int
eracts with the pathogen at the site of ingress to stimulate host defence e
nzymes. At high phosphite concentrations, phosphite acts directly on the pa
thogen to inhibit its growth before it is able to establish an association
with the host, and the host defences remain unchanged.