De. Swartz et al., Polymorphonuclear neutrophil (PMN) recruitment in a 2-front murine injury model: Triage of PMNs to competing stimuli of recruitment, SURGERY, 127(3), 2000, pp. 323-328
Background. Intensive care unit patients as a group ha-ire the highest rate
of nosocomial Infections, such as pneumonia, urinary tract infections, and
wound infections. The triage of polymorphonuclear neutrophils (PMNs) durin
g an acute inflammatory response was investigated to determine if the sever
ity of injury or infection contributes to PMN delivery.
Methods, A murine cecal ligation and puncture-induced peritonitis model wit
h polyvinyl sponge discs were used to collect the PMNs in the abdomen (prim
ary site) and in the subcutaneous tissue of the dorsum (remote site). Eight
y CD1 mab mice-20 in each of 4 groups-were assigned to the following: cecal
ligation and puncture (CLP), sham laparotomy with cecal manipulation (CM),
polyvinyl sponge placement in the abdomen and back only (SP), and sponge p
lacement in the back alone (CON [control]). After 24 hours, the sponges wer
e harvested and the PMNs were collected and counted on a hemocytometer.
Results. These data, reported as mean PMN cells x10(5) +/- SEM demonstrated
that back sponges contained significantly fewer PMNs in the CLP group (3.2
9 +/- 1.1) than in the CM group (7.77 +/- 1.61, P = .04), the SP group (8.6
9 +/- 1.67, P = .01), and the CON group (11.04 +/- 1.91, P < .001).
Conclusions. These results demonstrate that PMN delivery to sites of second
ary injury are inversely correlated to the severity of the primary injury o
r peritonitis.