Effects of air exchange, sucrose, and PPF on growth of Rehmannia glutinosaunder enriched CO2 concentration in vitro

The explants excised from Rehmannia glutinosa plantlets were cultured on Mu rashige-Skoog (MS) medium with or without sucrose under various environment al conditions such as air exchanges, photosynthetic photon flux (PPF) and C O2 enrichment. Healthy plantlets, which had normal stomatal activity and hi gh survival rate were obtained by increasing the air exchange rates per hou r in culture vessel. More than two times of leaf area and dry weight were o bserved compared with conventional tissue culture method under increased ai r exchange rate, high PPF and CO2 enriched conditions. Although the presenc e of sucrose led to the maximal growth, the plantlets cultured on sucrose-f ree medium showed fair growth characteristics. It was clearly shown that pl antlets grown under sucrosefree, CO2 enrichment and high PPF conditions had autotrophic growth characteristics.