Aj. Singer et al., Octylcyanoacrylate for the treatment of contaminated partial-thickness burns in swine: A randomized controlled experiment, ACAD EM MED, 7(3), 2000, pp. 222-227
Objective: To compare infection and reepithelialization rates of contaminat
ed second-degree burns treated with octylcyanoacrylate (OCA), silver sulfad
iazine (SSD), polyurethane (PU) film, and dry gauze (control; C) in swine.
Methods: Eighty standardized burns were created by applying an aluminum bar
preheated to 80 degrees C to the backs and flanks of young pigs for 20 sec
onds. All burns were immediately contaminated with 0.1 mt of Staphylococcus
aureus 10(5)/mL and randomly treated with OCA spray, SSD, PU, or dry gauze
(C). Full-thickness biopsies were taken at 3, 7, and 14 days for blinded h
istopathologic evaluation using hematoxylin and eosin (H&E)-stained slides.
Burns were considered infected in the presence of interstitial reticular d
ermal neutrophils containing bacteria (intraobserver agreement, K = 1.00).
Quantitative wound cultures were performed on a second day-3 specimen. Resu
lts: At day 3, wound infection rates were 30% (OCA), 50% (SSD), 55% (PU), a
nd 50% (C); p = 0.40 (Kruskal-Wallis test). At day 7, infection rates were
35% (OCA), 85% (SSD), 70% (PU), and 65% (C); p = 0.01. Median bacterial cou
nts at day 3 were 6,500 (OCA), 20,000 (SSD), 1,00,000 (PU), and 650,000 (C)
; p = 0.29. The proportion of completely reepithelialized wounds at day 14
were 75% (OCA), 90% (SSD), 85% (PU), and 90% (C); p = 0.50. Conclusions: Tr
eatment of contaminated partial-thickness burns with OCA spray resulted in
fewer infections at one week than with the other three treatments. Key word
s: burns; skin; partial thickness; bacterial contamination; infection; reep
ithelialization; healing; tissue adhesives; silver sulfadiazine; octylcyano
acrylate.