SCREENING THE PHYSICAL-PROPERTIES OF NOVEL PSEUDOMONAS EXOPOLYSACCHARIDES BY HPSEC WITH MULTI-ANGLE LIGHT-SCATTERING AND VISCOSITY DETECTION

The physical properties of three novel acidic exopolysaccharides obtai ned from P. marginalis types A, B and C, one from P. 'gingeri', one fr om P. andropogenis and one from P. fluorescens have been partially cha racterized. These EPSs were chromatographed on three serially placed S E Sheder OH pak columns covering a molar mass range for pullulans from about 4x10(7) to 1x10(3). The mobile phase was 0.05 M NaNO3. Physical measurements were performed on about 30 mg of sample for each EPS. Th e weight average molar mass of these EPSs ranged from about 0.71 to 2. 85x10(6), the weight average intrinsic viscosity from 7.15 to 35.3 dl/ g and the radius of gyration from 62 to 123 nm. The polydispersities o f these EPSs ranged from 1.01 to 1.37. The large molar mass, size and viscosities of these EPSs may indicate that they have potential for us e as thickeners, stabilizers, emulsifiers, and gelling agents in the f ood and non-food industries.