H. Kono et al., Medium-chain triglycerides inhibit free radical formation and TNF-alpha production in rats given enteral ethanol, AM J P-GAST, 278(3), 2000, pp. G467-G476
Citations number
50
Categorie Soggetti
da verificare
Journal title
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
This study determined whether free radical formation by the liver, tumor ne
crosis factor (TNF)-alpha production by isolated Kupffer cells, and plasma
endotoxin are affected by dietary saturated fat. Rats were fed enteral etha
nol and corn oil (E-CO) or medium-chain triglycerides (E-MCT) and control r
ats received corn oil (C-CO) or medium-chain triglycerides (C-MCT) for 2 wk
. E-CO rats developed moderate fatty infiltration and slight inflammation;
however, E-MCT prevented liver injury. Serum aspartate aminotransferase lev
els, gut permeability, and plasma endotoxin doubled with E-CO but were blun
ted similar to 50% with E-MCT. In Kupffer cells from E-CO rats, intracellul
ar calcium was elevated by lipopolysaccharide (LPS) in a dose-dependent man
ner. In cells from E-MCT rats, increases were blunted by similar to 40-50%
at all concentrations of LPS. The LPS-induced increase in TNF-alpha product
ion by Kupffer cells was dose dependent and was blunted by 40% by MCT. E-CO
increased radical adducts and was reduced similar to 50% by MCT. MCT preve
nt early alcohol-induced liver injury, in part, by inhibition of free radic
al formation and TNF-alpha production by inhibition of endotoxin-mediated a
ctivation of Kupffer cells.