Medium-chain triglycerides inhibit free radical formation and TNF-alpha production in rats given enteral ethanol

This study determined whether free radical formation by the liver, tumor ne crosis factor (TNF)-alpha production by isolated Kupffer cells, and plasma endotoxin are affected by dietary saturated fat. Rats were fed enteral etha nol and corn oil (E-CO) or medium-chain triglycerides (E-MCT) and control r ats received corn oil (C-CO) or medium-chain triglycerides (C-MCT) for 2 wk . E-CO rats developed moderate fatty infiltration and slight inflammation; however, E-MCT prevented liver injury. Serum aspartate aminotransferase lev els, gut permeability, and plasma endotoxin doubled with E-CO but were blun ted similar to 50% with E-MCT. In Kupffer cells from E-CO rats, intracellul ar calcium was elevated by lipopolysaccharide (LPS) in a dose-dependent man ner. In cells from E-MCT rats, increases were blunted by similar to 40-50% at all concentrations of LPS. The LPS-induced increase in TNF-alpha product ion by Kupffer cells was dose dependent and was blunted by 40% by MCT. E-CO increased radical adducts and was reduced similar to 50% by MCT. MCT preve nt early alcohol-induced liver injury, in part, by inhibition of free radic al formation and TNF-alpha production by inhibition of endotoxin-mediated a ctivation of Kupffer cells.