A method for application of samples to matrix-assisted laser desorption ionization time-of-flight targets that enhances peptide detection

Citation
F. Landry et al., A method for application of samples to matrix-assisted laser desorption ionization time-of-flight targets that enhances peptide detection, ANALYT BIOC, 279(1), 2000, pp. 1-8
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ANALYTICAL BIOCHEMISTRY
ISSN journal
00032697 → ACNP
Volume
279
Issue
1
Year of publication
2000
Pages
1 - 8
Database
ISI
SICI code
0003-2697(20000301)279:1<1:AMFAOS>2.0.ZU;2-0
Abstract
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry has become a fundamental tool for the identification and anal ysis of peptides and proteins. MALDI-TOF is well suited for the analysis of complex biological mixtures because samples are crystallized onto a solid support that can be washed to remove contaminants and salts prior to laser desorption. A number of approaches for immobilizing samples onto MALDI targ ets have been put forth. These include the use of different chemical matric es and the immobilization of samples onto different solid supports. In larg e part though, the preparation of MALDI targets has been an empirical exerc ise that often requires a unique series of conditions for every sample. Her e, a simple method for the application of peptide mixtures onto MALDI targe ts is put forth. This method differs because peptides are added directly to a sample of nitrocellulose dissolved in acetone, allowing them to interact in solution-phase organic solvent. This solution-phase mixture is then spo tted to the MALDI target and evaporated, forming a homogenous solid surface for laser desorption. This procedure is robust, highly sensitive, tolerant to detergents, and easily learned. In our hands, the method provides as mu ch as a 10-fold enhancement to the detection of tryptic peptide fragments d erived from in-gel digests. (C) 2000 Academic Press.