Physical map of a Plutella xylostella granulovirus genome

A plasmid library of restriction DNA fragments of a Plutella xylostella gra nulovirus (PxCV) was constructed, which covered all 9 EcoRI and all 19 Hind III fragments, and 18 of 20 PstI fragments. Restriction endonuclease analys is and DNA-DNA hybridization analysis of the cloned viral DNA fragments in the library and the restricted viral DNA fragments were carried out for con struction of a physical map of PxCV DNA for ApaI, BamHI, BglII, EcoRI, Hind III, KpnI, PstI, SacII, XbaI and XhoI. The size of PxGV genome was estimate d to be 102.2 kb. Location of the left-most DNA fragment on the physical ma p that contained granulin gene was determined by hybridization using a DNA probe of granulin gene of Trichoplusia ni granulovirus. Four homologous reg ions interspersed on the genome were also determined by DNA-DNA hybridizati on analysis.