Molecular cloning, tissue distribution and sequence analysis of complete glucokinase cDNAs from gilthead seabream (Sparus aurata), rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio)

The enzyme glucokinase (GK) (EC 2.7.1.1) plays an important role in the con trol of glucose homeostasis. Qualitative and/or quantitative variations in GK enzyme have been postulated by previous studies to explain why dietary c arbohydrate utilisation is lower in gilthead seabream (Sparus aurata) and r ainbow trout (Oncorhynchus mykiss) than in common carp (Cyprinus carpio). I n this study, we report the isolation and characterisation of a full-length cDNA coding for GK in these teleosts. Amino acid sequences derived from th ese cDNA clones are highly similar to other vertebrate GKs. These findings, including a detailed phylogenetic analysis, reveal that GK gene highly hom ologous to mammalian GK exists in these fish species with similar tissue sp ecific expression (mainly liver). (C) 2000 Elsevier Science B.V. All rights reserved.