Cloning and sequencing of the maltohexaose-producing amylase gene of Klebsiella pneumoniae

Authors
Momma, M
Citation
M. Momma, Cloning and sequencing of the maltohexaose-producing amylase gene of Klebsiella pneumoniae, BIOS BIOT B, 64(2), 2000, pp. 428-431
Citations number
12
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
2
Year of publication
2000
Pages
428 - 431
Database
ISI
SICI code
0916-8451(200002)64:2<428:CASOTM>2.0.ZU;2-L
Abstract
The molecular characterization of the maltohexaose-producing amylase gene o f Klebsiella pneumoniae revealed an open reading frame in which 2,031 base pairs encode a protein of 677 amino acids with a calculated molecular weigh t of 75,921, The amylase gene had high similarities of 73.6% in DNA sequenc e and 79.3% in deduced amino acid sequence with the periplasmic alpha-amyla se MalS gene of Escherichia coli.