Lysyl-tRNA synthetase of Bacillus stearothermophilus molecular cloning andexpression of the gene

Authors
Takita, T Shimizu, N Sukata, T Hashimoto, S Akita, E Yokota, T Esaki, N Soda, K Inouye, K Tonomura, B
Citation
T. Takita et al., Lysyl-tRNA synthetase of Bacillus stearothermophilus molecular cloning andexpression of the gene, BIOS BIOT B, 64(2), 2000, pp. 432-437
Citations number
23
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
64
Issue
2
Year of publication
2000
Pages
432 - 437
Database
ISI
SICI code
0916-8451(200002)64:2<432:LSOBSM>2.0.ZU;2-5
Abstract
The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA150 3 was cloned and sequenced. The gene consists of 1485 bp nucleotides commen cing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids, The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promo ter.