In the production of ethanol from lignocellulosics, large quantities o
f water are needed in various process steps. In industrial processes,
recycling of process streams is necessary to minimise fresh water requ
irements and decrease the amount of wastewater produced. This leads to
increases in various substances, both volatile and non-volatile which
inhibit fermentation. In the present Study ethanol was produced from
softwood in a bench-scale process development unit. The stillage strea
m from the distillation step was fractionated by evaporation and vario
us evaporation fractions characterised and their inhibitory effect on
fermentation investigated. irt was found that the volatile fractions h
ave no negative effect on the fermentation while the non-volatile frac
tion inhibited fermentation and resulted in a decreased yield. Simulat
ion of a six-effect evaporation unit based on the experimental data sh
ows that the intermediate evaporation condensate fractions are most su
ited for disposal. The chemical and biological oxygen demands are only
about 10% of the value in the stillage stream. Evaporation has been s
hown to be an effective way to drastically diminish the build-up of in
hibitory compounds when process streams are recirculated, but the ener
gy demand is high. Various process configurations to reduce the energy
demand are suggested. (C) 1997 Elsevier Science Ltd.