Anticlastogenic potential of 1 alpha,25-dihydroxyvitamin D-3 in murine lymphoma

Citation
A. Sarkar et al., Anticlastogenic potential of 1 alpha,25-dihydroxyvitamin D-3 in murine lymphoma, CANCER LETT, 150(1), 2000, pp. 1-13
Citations number
52
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER LETTERS
ISSN journal
03043835 → ACNP
Volume
150
Issue
1
Year of publication
2000
Pages
1 - 13
Database
ISI
SICI code
0304-3835(20000313)150:1<1:APO1AD>2.0.ZU;2-6
Abstract
Vitamin D-3, having gained scientific interest for so long because of its r ole in mineral homeostasis, has now received great importance as a possible antitumor agent. This study was undertaken in an attempt to visualize the possible anticlastogenic potential of the vitamin in an ascitic mouse lymph oma model namely, Dalton's lymphoma. Frequencies of structural type chromos omal aberrations, sister chromatid exchanges and micronucleus assays have b een chosen as the genotoxic endpoints in the proposed investigation. All th ese cytogenetic markers have been found to be markedly elevated during the progression of lymphoma in bone marrow cells. Vitamin D-3 effectively suppr essed the frequencies of chromosomal aberrations and sister chromatid excha nges in the lymphoma-bearing mice during the entire phase of tumor growth t hat significantly coupled with almost two-fold increase in survival time (3 7 +/- 2 and 68 +/- 2 days in lymphoma controls and vitamin D-3-treated lymp homa bearing mice, respectively), thus substantiating the antineoplastic ef ficacy of this secosteroid. The outcome of this study also is clearly refle cted in the depletion of circulating (serum) vitamin D-3 levels in the lymp homa control mice compared with normal (vehicle) controls while a still hig her level was maintained in the VD3-treated lymphoma mice. This anticlastog enic property of the vitamin has so far been neglected and this is the firs t attempt to unravel the vitamin D-3's effect in combating tumor developmen t in vivo by limiting the frequencies of chromosomal aberrations, sister ch romatid exchanges and micronuclei at least in transplantable murine model s tudied herein. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.