Purpose. To investigate the defensive effect of melatonin against oxidative
stresses in ultraviolet-B (UVB) radiation induced cataract development.
Methods. Young rats received 8 kJ/m(2) UVB for 15 min. For the intervention
of cataract development intraperitoneal injection of melatonin (4 mg/kg da
ily for 1 week) following UVB exposure was performed. Lenticular glutathion
e peroxidase (GSHPx), catalase (CAT), superoxide dismutase (SOD) activities
and glutathione (GSH), malondialdehyde (MDA) levels were determined in UVB
-melatonin, UVB, and control groups.
Results. One week after exposure in the UVB group, lens opacities were obse
rved and CAT, SOD, and GSHPx activities, and GSH level were lower than cont
rol and MDA level was higher than control (p < 0.05). In the UVB-melatonin
group CAT and SOD activities were lower than control (p < 0.05), and the MD
A level was lower than the UVB group (p < 0.05).
Conclusions. These results suggest that melatonin may protect against the U
VB-induced cataract development by directly quenching lipid peroxides and i
ndirectly by enhancing the production of the endogenous antioxidant GSH.