In 75 donkeys of Croatia that were classified into three types (Littoral-Di
naric, Northern-Adriatic and Istrian) transferrin and albumin polymorphisms
were studied by electrophoresis. Transferrin allele frequencies indicate t
he phylogenetic relationship of the Istrian and Northern-Adriatic type of d
onkey, as well as the significant difference of Littoral-Dinaric type in re
lation to populations living in the area of Kvarner islands and Istria. Alb
umin allele frequencies have not indicated a significant difference between
the types. Standard genetic distance is the greatest between Littoral-Dina
ric and Istrian types and the smallest between Northern-Adriatic and Istria
n types of donkey, which also corresponds to the geographical distance of t
he areas in which these populations live. In order to get more information,
it is necessary to direct further research towards determination of freque
ncies of other polymorphic proteins and direct DNA typing.