NUCLEASE SUSCEPTIBILITY OF THE RAT-LIVER SATELLITE DNA-CONTAINING CHROMATIN DECREASES WITH AGE

Authors
THAKUR MK CHAURASIA P
Citation
Mk. Thakur et P. Chaurasia, NUCLEASE SUSCEPTIBILITY OF THE RAT-LIVER SATELLITE DNA-CONTAINING CHROMATIN DECREASES WITH AGE, Molecular and cellular biochemistry, 171(1-2), 1997, pp. 45-48
Citations number
17
Categorie Soggetti
Biology,"Cell Biology
Journal title
Molecular and cellular biochemistry → ACNP
ISSN journal
03008177
Volume
171
Issue
1-2
Year of publication
1997
Pages
45 - 48
Database
ISI
SICI code
0300-8177(1997)171:1-2<45:NSOTRS>2.0.ZU;2-I
Abstract
Nuclease susceptibility of the satellite DNA-containing chromatin of t he liver of young (18 +/- 2 weeks) and old (100 +/- 5 weeks) rats was analysed using nick-translated rat 185 bp satellite I DNA fragment clo ned in pBR322. With increasing concentration of DNaseI and micrococcal nuclease (MNase), multimeric forms of the satellite ladder gradually disappear in both the ages. The rate of disappearance is faster in you ng rats as compared to old ones. Such age-dependent decrease in the su sceptibility of satellite DNA-containing chromatin reflects its conden sation towards heterochromatization in old age.