Vl. Kumar et al., ANDROGEN DEPRIVATION CAUSES UP-REGULATION OF ANDROGEN RECEPTOR TRANSCRIPT IN THE RAT PROSTATE, Molecular and cellular biochemistry, 171(1-2), 1997, pp. 133-138
We have studied the effect of androgenic deprivation on the level of a
ndrogen receptor transcript in the rat ventral prostate. The rats were
treated with estradiol benzoate, flutamide and [D Trp(6), des Gly(10)
]gonadotropin releasing hormone (GnRH) for different time periods. The
se treatments produced a significant decrease in the weight of prostat
e. Total RNA isolated from the ventral prostates was hybridized with t
he cDNA probe for androgen receptor. Densitometric analysis of the aut
oradiographic signal revealed a rise in the level of androgen receptor
RNA following treatment of rats with estradiol benzoate and flutamide
. Treatment of rats with [D Trp(6), des Gly(10)] GnRH brought about a
transient rise in the level of androgen receptor RNA. Thus, our result
s indicate that androgenic deprivation up-regulates the level of andro
gen receptor transcript.