Separation of D-lysergic acid diethylamide derivatives using micellar electrokinetic capillary chromatography

By adjusting column temperature and applied electric field, a fast separati on in micellar electrokinetic capillary chromatography was developed for th e separation of D-lysergic acid diethylamide derivatives. A baseline separa tion of nine derivatives was accomplished with a run time of less than 12 m in by utilizing elevated column temperature (60 degrees C) and an applied e lectric field of 387 V/cm. The number of plates generated per unit time for the separations completed at elevated temperatures was significantly highe r when compared to separations at the same applied electric field but at lo wer temperatures (20 degrees C).