Molecular cloning of fresh water and deep-sea rod opsin genes from Japanese eel Anguilla japonica and expressional analyses during sexual maturation

Authors
Zhang, H Futami, K Horie, N Okamura, A Utoh, T Mikawa, N Yamada, Y Tanaka, S Okamoto, N
Citation
H. Zhang et al., Molecular cloning of fresh water and deep-sea rod opsin genes from Japanese eel Anguilla japonica and expressional analyses during sexual maturation, FEBS LETTER, 469(1), 2000, pp. 39-43
Citations number
30
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
469
Issue
1
Year of publication
2000
Pages
39 - 43
Database
ISI
SICI code
0014-5793(20000303)469:1<39:MCOFWA>2.0.ZU;2-H
Abstract
We have determined the complete cDNA sequences of fresh water rod opsin gen e (fwo) and deep-sea rod opsin gene (dso) from Japanese eel Anguilla japoni ca. The cDNA clones of fwo and dso consisted of 1437 and 1497 nucleotides, respectively. The predicted opsins of both genes consisted of 352 amino aci d residues. Southern blot and PCR analyses of genomic DNA indicated that th e Japanese eel genome contains only one fwo and one dso and they are intron less. Quantitative RT-PCR analyses revealed that the expression of fwo decr eases with sexual maturation while that of rise increases. (C) 2000 Federat ion of European Biochemical Societies.