One-codon alternative splicing of the CpG MTase Dnmt1 transcript in mouse somatic cells

Citation
Mj. Lin et al., One-codon alternative splicing of the CpG MTase Dnmt1 transcript in mouse somatic cells, FEBS LETTER, 469(1), 2000, pp. 101-104
Citations number
29
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
469
Issue
1
Year of publication
2000
Pages
101 - 104
Database
ISI
SICI code
0014-5793(20000303)469:1<101:OASOTC>2.0.ZU;2-A
Abstract
The genomic methylation patterns in the mammalian somatic cells are presuma bly maintained by a single enzyme, dnmt1, In mouse, this DNA (cytosine-5)-m ethyltransferase, or CpG MTase, is encoded by the Dnmt1 gene. We now presen t evidence that in different tissues and cell types, the primary transcript of mouse dnmt1 is alternatively spliced to generate two poly-(A) RNAs of a pproximately similar abundance, This alternative splicing most likely origi nates from the existence of two tandemly arranged acceptor sites separated by only 3 nt. The two Dnmt1 mRNAs thus encode two CpG MTases differing by t wo amino acids, We discuss the implications of the discovery of two dnmt1 i sozymes, instead of one enzyme as previously thought, in the somatic cells of both mouse and human. (C) 2000 Federation of European Biochemical Societ ies.