Mh. Moh et al., Improved separation of sucrose ester isomers using gradient high performance liquid chromatography with evaporative light scattering detection, FOOD CHEM, 69(1), 2000, pp. 105-110
A reversed-phase high performance liquid chromatography (HPLC) with evapora
tive light scattering detection is described for the analysis of commercial
sucrose esters. The binary gradient consists initially of 75% methanol and
25% water. After 70 min, the ratio is changed to 95% methanol and 5% water
. The gradient is then reverted back to the initial ratio after 120 min for
another injection. This procedure provides a complete separation of monoes
ters and diesters with different acyl chain lengths (C-16 and C-18). The mo
noester analyzed was resolved into 3-6 peaks and diesters into 14-18 peaks.
The identities of monoesters with various acyl chain lengths were further
established by liquid chromatography mass spectrometry (LCMS). This HPLC pr
ocedure has several advantages over other reported methods in terms of impr
oved resolution and simultaneous separation of mono- and diesters. With thi
s method it is, thus, possible to determine the approximate composition of
mono- and diester of sucrose esters in one analysis. (C) 2000 Elsevier Scie
nce Ltd. All rights reserved.