Chromosomal aberrations evaluated by CGH, FISH and GTG-banding in a case of AIDS-related Burkitt's lymphoma

Background and Objectives, We have previously reported on a complex chromos ome rearrangement [der(17)] in a B-cell line, uno A, established from an _A IDS patient with Burkitt's lymphoma (BL), The aim of the present study was the definition of der(17) composition and the identification of complete or partial chromosome gains and losses In two cell clones (BRG A and BRG M) d erived from this patient. Design and Methods, We applied comparative genome hybridization (CGH) to de tect the DNA mis-representations in the genome of the two cell clones. Find ings from CGH and banding analysis could then direct the choice of probes f or chromosome painting experiments to elucidate der(17) composition. Results, CGH analysis identified gains of chromosomes 1q, 7q, 12q, 13q, 15q , 17p, 20p,q and losses of chromosomes 3p and 5q in BRG A and gain of chrom osome 1q and loss in chromosome 6q In BRG M. Some of the detected alteratio ns had already been described in lymphomas, while others appeared to be new . The combination of these techniques allowed a precise definition of der(1 7), composed by translocated regions from chromosomes 12 and 15, Interpretation and Conclusions, We demonstrated CGH to be a powerful tool I n the Identification of recurrent chromosome aberrations In an AIDS-related at. and in ascertaining the origin of marker chromosomes. We were also abl e to identify a different pattern of aberrations and assess an independent sequence of events leading to the Ip gain In the two subclones. (C) 2000, F errata Storti Foundation.