The expression of three types of CINCs by lipopolysaccharide-stimulated rat macrophages is inhibited similarly by anti-inflammatory steroids

Objective: Expression of CINCs was regulated differentially in lipopolysacc haride-stimulated rat macrophages. We examined whether the expression of CI NCs in lipopolysaccharide-stimulated rat macrophages is similarly inhibited by anti-inflammatory drugs. Materials and Methods: Rat peritoneal macrophages were stimulated by lipopo lysaccharide in the presence of antiinflammatory steroids (dexamethasone, p rednisolone and hydrocortisone) or non-steroidal anti-inflammatory drugs (i ndomethacin and piroxicam). The production and mRNA expression of three typ es of CINCs were measured using enzyme-linked immunosorbent assay and north ern hybridization. Results: Anti-inflammatory steroids; dexamethasone, prednisolone and hydroc ortisone, dose-dependently inhibited the production of CINC-1, -2 and -3, w hose inhibitory patterns were similar to each other. Furthermore mRNA expre ssion of each CINC was inhibited by dexamethasone in a dose-dependent manne r. In contrast, non-steroidal anti-inflammatory drugs, indomethacin and pir oxicam were without effect. Expression of each CINC was regulated different ly; the production of CINC-1 reached a maximum at 12 h and then slightly de creased after lipopolysaccharide stimulation, whereas that of CINC-2 and CI NC-3 increased up to 24 h. Dexamethasone inhibited the CINCs production and mRNA. expression at 9 h after lipopolysaccharide stimulation. Conclusions: These results indicate no difference among CINC-1, -2 and -3 i n the inhibition of production and mRNA expression of CINCs by anti-inflamm atory steroids, although lipopolysaccharide differentially induces expressi on of each CINC expression in culture of rat macrophages.