O. Osmanagaoglu et al., Cloning and expression of a plasmid-linked pediocin determinant trait of Pediococcus acidilactici F, J BASIC MIC, 40(1), 2000, pp. 41-49
Plasmid DNA from Pediococcus acidilactici F was prepared by lysozyme-mutano
lysin method and purified by cesium chloride-ethidium bromide (CsCl-EtBr) d
ensity gradient ultracentrifugation. Agarose gel electrophoresis of plasmid
DNA and plasmid-curing experiments suggested that bacteriocin activity was
harboured on a small plasmid of approximately 9.1 kb (kilobasepair) in Ped
iococcus acidilactici F. Plasmid encoding bacteriocin production in P. acid
ilactici F was examined for restriction enzyme cleavage patterns and its ma
p has been constructed. An Escherichia coli strain transformed with the rec
ombinant plasmid, pQE322, produced and, most probably, secreted pediocin F.