J. Hollender et al., Biomonitoring of environmental polycyclic aromatic hydrocarbon exposure bysimultaneous measurement of urinary phenanthrene, pyrene and benzo[a]pyrene hydroxides, J CHROMAT B, 739(1), 2000, pp. 225-229
A high-performance liquid chromatographic method with fluorescence detectio
n was developed which enables the simultaneous determination of the urinary
polycyclic aromatic hydrocarbon metabolites 3-hydroxyphenanthrene, 1-hydro
xypyrene and 3-hydroxybenzo[a]pyrene. The method has small solvent consumpt
ion because of the use of a microbore RP C-18 column and a relatively short
run time. Low detection limits of 0.02 nmol/l for 3-hydroxypyrene to 0.19
nmol/l for 3-hydroxybenzo[a]pyrene were attained. In contrast, the detectio
n limits of alpha-naphthol and 9,10-dihydroxy-9,10-dihydrophenanthrene were
not adequate for the determination of environmental exposure. The develope
d method was successfully used for the analysis of urine samples from child
ren. (C) 2000 Elsevier Science B.V. All rights reserved.