HYPERTONIC-HYPEROSMOTIC SOLUTION MODIFIES MYOGLOBIN LEVELS IN EARLY REPERFUSION AFTER ISCHEMIC CARDIAC-ARREST EXPERIMENTAL-MODEL

To evaluate the effect of reperfusion with hypertonic-hyperosmotic sol ution, cardiectomy was performed in 25 New Zealand white rabbits. Seve n isolated hearts were submitted to 30 min of global ischemia and repe rfused with oxygenated buffer for 60 min. Myoglobin and isoenzyme MB o f creatine kinase concentrations were each measured in the effluent 15 min, and values were correlated (r = 0.5011, p = 0.015). After this p rocedure, 18 isolated hearts were randomized in two groups. Hearts of group I were reperfused with hypertonic-hyperosmotic solution (NaCl 7. 5% dextran 60,000 MW) diluted in oxygenated buffer, and group II with oxygenated buffer. Myoglobin and coronary flow were measured in both g roups, group I showed lower levels of myoglobin (p = 0.0069) and highe r coronary flow (p = 0.028) than group II. In conclusion, changes in m yoglobin concentration in the heart effluent are more sensitive than c hanges in isoenzyme MB of creatine kinase; thus, evaluation of this pa rameter may be useful in the detection of ischemia reperfusion injury. Additionally, hypertonic-hyperosmotic solution improves the coronary now and has a protective effect against ischemia-reperfusion injury.