The dendritic cells (DC) of mouse spleen and thymus were examined for expre
ssion of CD4 and CD8, Provided care was taken to avoid selective extraction
or selective depletion of DC subpopulations, three main types of DC were d
etected in mouse spleen: a major new population of CD4(+)8(-) DEC-205(low)
CD11b(high) DC, together with the previously described CD4(-)8(-) DEC-205(l
ow) CD11b(high) DC and CD4(-)8 alpha alpha(+) DEC-205(high) CD11b(low) DC.
The CD4 on the surface of the CD4(+) splenic DC subpopulation was produced
by the DC themselves, and CD4 RNA transcripts were present. Likewise, the C
D8 alpha on the surface of the splenic CD8(+) DC was shown to be a product
of the DC themselves, in agreement with earlier evidence, All three spleen
DC types would be considered as mature, based on expression of CD80, CD86,
and CD40 as well as on T cell stimulating function, Mouse thymuses appeared
to contain two DC types; both were DEC-205(high)CD11b(low), but they diffe
red in the level of CD8 alpha alpha expression. However, as well as this au
thenticated marker expression, immunofluorescent staining was also found to
reflect a series of artifacts, due to the autofluorescence of contaminatin
g cells and due to pickup of CD4 and CD8 alpha beta. By constructing mice c
himeric for the hemopoietic lineages using mixtures of wild-type bone marro
w with CD4(null) or CD8 alpha(null) bone marrow, a marked pickup by thymic
DC of Ags derived from thymocytes was demonstrated.