Sixteen healthy subjects were intravenously injected with lipopolysaccharid
e (LPS), once with placebo and once with recombinant human interleukin OL)-
10 (25 mu g/kg), to determine the effect of IL-10 on LPS-induced production
of macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta, and monocyte
chemoattractant protein (MCP)-1. LPS induced transient increases in serum
MIP-1 alpha, MIP-1 beta, and MCP-1. Pretreatment with IL-10 inhibited LPS-i
nduced release of MIP-1 alpha, MIP-1 beta, and MCP-1, Ln whole blood in vit
ro, the IL-10-induced inhibition of MTP-1 alpha and MIP-1 beta release was
equally potent in the presence or absence of an anti-tumor necrosis factor
(TNF) antibody. Although isolated peripheral blood mononuclear cells produc
ed more MIP-1 alpha and MIP-1 beta than neutrophils, the latter cells were
more sensitive to the inhibiting effect of IL-10, IL-10 attenuates LPS-indu
ced production of CC chemokines in human endotoxemia, whereby in vitro expe
riments suggest that, in the case of MIP-1 alpha and MIP-1 beta release, th
is effect is independent from an inhibitory effect on TNF production.