An improved HPLC method for identification and quantitation of polyamines and related compounds as benzoylated derivatives

A simple reversed phase HPLC method was developed for the determination of polyamines (putrescine, cadaverine, spermidine, spermine) and other polycat ions (n-acetyl putrescine, histamine, n-acetilspermine, agmatine), as their benzoylated derivatives. The advantages of this method, examined in variou s matrixes (hypothalamus, hypophyses, ovary, and seminal plasma), are its v ersatility, simplicity, and short time of determination of polyamines and r elated substances of importance in prokaryotes and eukaryotes, even in diff erent matrixes, using a standard HPLC equipment (UV detection at 254 nm), w ith good resolution, sensitivity, and reproducibility.