Determination of furosine in hydrolyzate of processed milk by HPLC using anarrow bore column and diode-array detector

Authors
Nicoletti, I Cogliandro, E Corradini, C Corradini, D Pizzoferrato, L
Citation
I. Nicoletti et al., Determination of furosine in hydrolyzate of processed milk by HPLC using anarrow bore column and diode-array detector, J LIQ CHR R, 23(5), 2000, pp. 717-726
Citations number
12
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
10826076 → ACNP
Volume
23
Issue
5
Year of publication
2000
Pages
717 - 726
Database
ISI
SICI code
1082-6076(2000)23:5<717:DOFIHO>2.0.ZU;2-P
Abstract
A simple, rapid, sensitive, reproducible, and accurate HPLC procedure emplo ying a narrow bore reversed phase column and a diode-array detector is prop osed for the determination of epsilon-N-2-furoylmethyl-L-lysine (furosine) in acid hydrolyzed of milk and powdered milk samples. After optimization of the variables involved, the method was characterized and validated in terms of selectivity, repeatability, sensitivity, and accu racy and applied to determine furosine content in hydrolyzed milk samples.