PRODUCTION OF COMMERCIAL LILIUM-RUBELLUM BAKER BULBS - EFFECTS OF VOLUME AND RENEWAL OF LIQUID-MEDIUM ON IN-VITRO GROWTH, BULB ROT INFECTION DURING COLD TREATMENT, AND POST-IN-VITRO GROWTH OF BULBLETS
Y. Niimi et al., PRODUCTION OF COMMERCIAL LILIUM-RUBELLUM BAKER BULBS - EFFECTS OF VOLUME AND RENEWAL OF LIQUID-MEDIUM ON IN-VITRO GROWTH, BULB ROT INFECTION DURING COLD TREATMENT, AND POST-IN-VITRO GROWTH OF BULBLETS, Engei Gakkai Zasshi, 66(1), 1997, pp. 113-119
Bulblets of Lilium rubellum Baker were cultured for 16 weeks in a 20-,
30- or 40-ml liquid medium renewed 0, 1, or 3 times. The bulblets wer
e assessed for growth and rot infection. 1. Bulblets with fresh weight
of more than 600 mg were obtained by using a 20- or 30-ml medium with
3 renewals, or 40 ml with one renewal. 2. Bulblets frequently turned
brown when they were cultured in a 30- or 40-ml medium with 3 renewals
; they frequently rotted both during a cold treatment and after transp
lantation. 3. Bulblets cultured in a 20-ml medium did not rot after a
cold treatment irrespective of the number of medium renewals; they gre
w well after transplantation to the greenhouse. 4. Bulblets cultured i
n a 20-ml medium with 3 renewals most frequently developed into plantl
ets with elongated axes 15 weeks after transplantation. These results
indicate that the 20-ml medium with 3 renewals appears to be most suit
able for bulblet culture of L. rubellum in a liquid medium.