Doubled haploid development and PCR-analysis of F-1 hybrid derived DH-R-2 paprika (Capsicum annuum L.) lines

Anther derived spontaneous doubled haploid (DH-R-1) plants were developed f rom an intercultivar F-1 hybrid (Holland) of <blocky> type pepper cv. <Mazu rka> (Capsicum annuum L. var. annuum, 2n = 24). Regenerants were grown in g reenhouses for seed production to R-2-generation (DH-R-2) prior to flow cyt ometric analysis which revealed DH-R-1 plants with haploid (n = 12); double d haploid, DH, (2n = 24); tetraploid (2n = 48); and aneuploid genomes. Poll en grains of anther cultures showed a high decrease in viability during the first two weeks of incubation time. The DNA samples of the 47 DH-R-2 indiv iduals were bulked in nine groups and subjected to PCR analysis for estimat ing the level of homogeneity versus heterogeneity in comparison to the cont rol of anther donor bulk of five plants of F-1 hybrid pepper cv. <Mazurka> (DH-R-0). in total 26 primers were applied, 22 of them revealed scorable PC R profiles with 54 scorable PCR bands. Of these, 19 (35.2%) bands generated by 7 primers were polymorphic. All of the nine bulks of DH-R-2 plane were discriminated at least by one primer.