Media composition and anther plating for production of androgenetic green plants from cultivated rye (Secale cereale L.)

Media composition and anther plating environment were studied in rye anther culture. In the studies on media the effects of basal media composition, p lant growth regulators, glutamine, sucrose and maltose, and solidification of media were investigated. Increase in the size of the induction dish impr oved regeneration of green plants. The basal media W14 and 190-2 were super ior in promoting callus induction and green plant regeneration. Genotype by medium interactions for induction were not recorded. Dicamba auxin in the induction medium seemed beneficial for the green to albino plant ratio. Glu tamine improved induction generally and green plant regeneration with a res ponsive genotype. Adding 10% ficoll type 400 was favourable for induction w ith one rye line, but this effect was not confirmed in subsequent testing w ith two different ryes. Solid medium promoted the highest production of gre en plants. Maltose in induction medium was superior to glucose and the malt ose concentration of 6% seemed optimal for induction in comparison with hig her concentrations and most cost-effective for production of green plants.