POSSIBILITY OF CLASSIFICATION IN SOME SPECIES OF LILIUM BY PCR-RFLP OF RIBULOSE-1, 5-BISPHOSPHATE CARBOXYLASE LARGE SUBUNIT (RBCL) GENE ANDRIBOSOMAL-RNA GENE

PCR-RFLP analysis was applied to classify nine Lilium species, L. japo nicum, L. rubellum, L. nobilissimum, L. speciosum, L. auratum, L. aura tum var. platyphyllum, L. henryi, L. concolor var. pulchellum and L. l eichtlinii var. maximowiczii, and one interspecific hybrid, L. x formo longi. When PCR products amplified in specific regions of ribulose-1, 5-bisphosphate carboxylase large subunit (rbcL) gene in chloroplast an d nuclear ribosomal RNA (rRNA) gene were digested by 20 restriction en zymes, some polymorphisms were observed. The examined plants were clas sified into six groups based on the combined results in rbcL and rRNA gene. The tested species in Archelirion and Sinomartagon were classifi ed into different groups. Furthermore six species in Archelirion were classified into three groups. Thus our study suggested that PCR-RFLP a nalysis could be used for classification of lilies.