M. Pavlica et al., Detection of micronuclei in haemocytes of zebra mussel and great ramshorn snail exposed to pentachlorophenol, MUT RES-GTE, 465(1-2), 2000, pp. 145-150
Citations number
39
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
The frequency of micronuclei (MN) induced by pentachlorophenol (PCP) in hae
mocytes of zebra mussel, Dreissena polymorpha Pall. and great ramshorn snai
l, Planorbarius corneus L. was determined over a 14 days of exposure (sampl
ing after 4, 7 and 14 days) under laboratory conditions, PCP doses for zebr
a mussel ranged from 10 to 150 mu g/l, and for ramshorn snail from 10 to 45
0 mu g/l. Micronuclei were detected after bisbenzimide fluorescent staining
. Positive responses were observed in both species. The mean MN frequencies
in treated mussels ranged between 0.69 and 7.50 parts per thousand, and be
tween 2.07 and 13.80 parts per thousand in treated snails. The spontaneous
MN levels in mussels averaged from 0.5 to 2.75 parts per thousand, and in s
nails from 1.55 to 2.00 parts per thousand. Our results suggest that haemol
ymph of both species represent an appropriate test tissue in environmental
genotoxicity assessment. (C) 2000 Elsevier Science B.V. All rights reserved
.