Expression of Ki-67, tumor suppressor proteins, growth factor, and growth factor receptor in juvenile respiratory papillomatosis: Ki-67 and p53 as predictors of aggressive disease
Y. Stern et al., Expression of Ki-67, tumor suppressor proteins, growth factor, and growth factor receptor in juvenile respiratory papillomatosis: Ki-67 and p53 as predictors of aggressive disease, OTO H N SUR, 122(3), 2000, pp. 378-386
The enhanced proliferation of epithelial cells is a typical feature of resp
iratory papilloma. the mechanism or mechanisms leading to abnormal epitheli
al proliferation remain unclear. Overexpression of growth factors and their
receptors and inactivation of tumor-suppressor proteins are known to cause
cell transformation and proliferation. The objectives of this study were t
o evaluate the expression of these factors in juvenile respiratory papillom
as with correlation to cellular proliferation activity, and to determine wh
ether such expression is associated with the clinical course of the disease
. The expression of transforming growth factor-alpha, epidermal growth fact
or receptor, p53 protein, retinoblastoma proteins and Ki-67 was quantified
by immunohistochemistry in paraffin-embedded biopsy specimens taken at the
initial surgical excision from children in whom respiratory papillomatosis
was diagnosed. Clinical information regarding the number of disease sites,
tracheobronchial spread, malignant transformation, and frequency of recurre
nces was reviewed. Thirty-five specimens were suitable for immunohistochemi
cal evaluation. Ki-67 expression was significantly higher in patients with
multiple sites of disease and frequent recurrences. High p53 expression was
significantly associated with malignant transformation. We concluded that
Ki-67 and p53 expression may be predictive of the clinical course in childr
en with respiratory papillomatosis.