By using radioimmunoassays toward the cDNA-predicted amino acid sequence of
human glucagon-like peptide-2, a peptide was isolated from extracts of hum
an ileum. By mass spectrometry and Edman sequencing, this peptide was ident
ified as human proglucagon 126-158. High-performance liquid chromatography
analyses indicated that a similar immunoreactive peptide (iGLP-2) was prese
nt in human plasma. Human plasma concentrations of iGLP-2 were elevated 3-
to 4-fold at 1 to 2 h after ingestion of 800 to 1200 kcal meals. (C) 2000 E
lsevier Science Inc. All rights reserved.