The pilL and pilN genes of Incl1 plasmids R64 and Collb-P9 encode outer membrane lipoproteins responsible for thin pilus biogenesis

Authors
Sakai, D Komano, T
Citation
D. Sakai et T. Komano, The pilL and pilN genes of Incl1 plasmids R64 and Collb-P9 encode outer membrane lipoproteins responsible for thin pilus biogenesis, PLASMID, 43(2), 2000, pp. 149-152
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
PLASMID
ISSN journal
0147619X → ACNP
Volume
43
Issue
2
Year of publication
2000
Pages
149 - 152
Database
ISI
SICI code
0147-619X(200003)43:2<149:TPAPGO>2.0.ZU;2-1
Abstract
The predicted amino acid sequences of the pill and pilN genes, required for the thin pilus formation of IncI1 plasmids R64 and ColIb-P9, contain N-ter minal lipoprotein signal peptide motifs. The pill and pilN products were la beled with [H-3]palmitic acid as 38- and 57-kDa proteins, respectively, ind icating that they are lipoproteins. Both PilL and PilN were localized to th e outer membrane, (C) 2000 Academic Press.