Novel process for enzymatic hydrolysis of proteins in an aqueous two-phasesystem for the production of peptide mixture

Authors
Murakami, Y Hirata, A
Citation
Y. Murakami et A. Hirata, Novel process for enzymatic hydrolysis of proteins in an aqueous two-phasesystem for the production of peptide mixture, PREP BIOC B, 30(1), 2000, pp. 31-37
Citations number
12
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
ISSN journal
10826068 → ACNP
Volume
30
Issue
1
Year of publication
2000
Pages
31 - 37
Database
ISI
SICI code
1082-6068(2000)30:1<31:NPFEHO>2.0.ZU;2-S
Abstract
A novel process for the production of peptide mixtures is proposed. Biologi cally active peptides were synthesized using a thermolysin-catalyzed hydrol ysis of a corn protein (zein) in an aqueous two-phase system. The mixture o f-peptides which was selectively recovered from the dextran-rich bottom pha se had a higher angiotensin-converting enzyme (ACE) inhibitory activity tha n native zein.