Preliminary study on the cleavage of fusion protein GST-CMIV with palladium(II) complex

Authors
Dou, F Qiao, F Hu, JZ Zhu, TY Xu, XX Zhu, DX Zhu, LG
Citation
F. Dou et al., Preliminary study on the cleavage of fusion protein GST-CMIV with palladium(II) complex, PREP BIOC B, 30(1), 2000, pp. 69-78
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
ISSN journal
10826068 → ACNP
Volume
30
Issue
1
Year of publication
2000
Pages
69 - 78
Database
ISI
SICI code
1082-6068(2000)30:1<69:PSOTCO>2.0.ZU;2-P
Abstract
A novel method for post-treatment of gene-engineered proteins is reported. A coden of Cys-His unit is introduced into the N-terminal of cecropin CMIV by using PCR. The gene is expressed in E. coil fused with GST. After purifi cation, the fusion protein is cleaved by [Pd(en)(H2O)(2)](2+) at the His-Ar g bond and the cecropin CMIV with antibacterial activity is obtained. The p reliminary results held some promise of success for application of the pall adium(II) complex as cleavage agent for the production of peptide drugs fro m gene-engineering fusion proteins.