Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida

The outer membrane protein of Oma87 from Pasteurella multocida A:1 has sign ificant similarity to the D15 protective antigen of Haemophilus influenzae (Ruffolo and Adler, 1996). Four fragments of Oma87 from a P. multocida sero type D strain were cloned into a pGEX expression vector and transformed int o E. coli JM105. Western blot analysis revealed that convalescent chicken s era reacted with only GST-F1 fusion protein which contained amino acids 18 through to 130 of Oma87 fused to the GST protein. Vaccination with the GST- F1 protein failed to protect chickens against challenge with a virulent P. multocida serotype A. (C) 2000 Elsevier Science B.V. All rights reserved.