Participation of the tightly-bound (putative cytoskeleton-bound) polysomesin translation during germination of dormant and non-dormant cereal caryopses

Research was done on dormant and non-dormant barley cv. Ars caryopses and t riticale cv. Grade caryopses treated and non-treated with abscisic acid (AB A). During germination higher participation of populations of so-called tig htly-bound polysomes (TBP) in embryos of dormant barley caryopses was obser ved, as well as their high metabolic activity. In embryos of triticale cary opses of which dormancy was imposed in an artificial way by ABA (100 mu M), the strongest incorporation of C-14-amino acids into nascent polypeptide c hains in vivo was found in population of TBP, as well as the highest partic ipation among three of the studied fractions (free polysomes, membrane-boun d polysomes ind tightly-bound polysomes). These results may indicate the si gnificant role of TBP (putative cytoskelelon-bound polysomes - CBP) in main taining dormancy during imbibition of cereal caryopses.