HUMAN CYTOMEGALOVIRUS INDUCES INTERLEUKIN-8 PRODUCTION BY A HUMAN MONOCYTIC CELL-LINE, THP-1, THROUGH ACTING CONCURRENTLY ON AP-1-BINDING AND NF-KAPPA-B-BINDING SITES OF THE INTERLEUKIN-8 GENE

Cytomegalovirus (CMV) infection induced interleukin-8 (IL-S) gene tran scription in a human monocytic cell line, THP-1 cells, leading to IL-S secretion, The functional analysis of the IL-X gene revealed that bot h AP-1-and NF-kappa E factor-binding elements were involved in conferr ing the responsiveness to CMV. Moreover, electrophoretic mobility shif t assays demonstrated that CMV induced the formation of NF-kappa B and AP-1 complexes. These results suggest that CMV activates these transc riptional factors, resulting in IL-8 gene expression.